Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA.

Authors :: Iaccarino N
Cheng M
Qiu D
Pagano B
Amato J
Di Porzio A
Zhou J
Randazzo A
Mergny JL
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2021 Apr 26; Vol. 60 (18), pp. 10295-10303. Date of Electronic Publication: 2021 Mar 24.
Publication Year :: 2021
Abstract: The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine-cytosine <superscript>+</superscript> (C:C <superscript>+</superscript> ) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.<br /> (© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)