Back to Search
Start Over
Immunopathogenic CSF TCR repertoire signatures in virus-associated neurologic disease.
- Source :
-
JCI insight [JCI Insight] 2021 Feb 22; Vol. 6 (4). Date of Electronic Publication: 2021 Feb 22. - Publication Year :
- 2021
-
Abstract
- In this study, we examined and characterized disease-specific TCR signatures in cerebrospinal fluid (CSF) of patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). TCR β libraries using unique molecular identifier-based methodologies were sequenced in paired peripheral blood mononuclear cells (PBMCs) and CSF cells from HAM/TSP patients and normal healthy donors (NDs). The sequence analysis demonstrated that TCR β repertoires in CSF of HAM/TSP patients were highly expanded and contained both TCR clonotypes shared with PBMCs and uniquely enriched within the CSF. In addition, we analyzed TCR β repertoires of highly expanded and potentially immunopathologic HTLV-1 Tax11-19-specific CD8+ T cells from PBMCs of HLA-A*0201+ HAM/TSP and identified a conserved motif (PGLAG) in the CDR3 region. Importantly, TCR β clonotypes of expanded clones in HTLV-1 Tax11-19-specific CD8+ T cells were also expanded and enriched in the CSF of the same patient. These results suggest that exploring TCR repertoires of CSF and antigen-specific T cells may provide a TCR repertoire signature in virus-associated neurologic disorders.
- Subjects :
- CD8-Positive T-Lymphocytes
Clone Cells
HTLV-I Infections
Human T-lymphotropic virus 1
Humans
Leukocytes, Mononuclear
Nervous System Diseases virology
Paraparesis, Tropical Spastic blood
Receptors, Antigen, T-Cell immunology
Receptors, Antigen, T-Cell, alpha-beta blood
Nervous System Diseases immunology
Paraparesis, Tropical Spastic cerebrospinal fluid
Paraparesis, Tropical Spastic immunology
Receptors, Antigen, T-Cell, alpha-beta immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2379-3708
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- JCI insight
- Publication Type :
- Academic Journal
- Accession number :
- 33616082
- Full Text :
- https://doi.org/10.1172/jci.insight.144869