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Validation of molecular response of tuberization in response to elevated temperature by using a transient Virus Induced Gene Silencing (VIGS) in potato.
- Source :
-
Functional & integrative genomics [Funct Integr Genomics] 2021 Mar; Vol. 21 (2), pp. 215-229. Date of Electronic Publication: 2021 Feb 21. - Publication Year :
- 2021
-
Abstract
- Temperature plays an important role in potato tuberization. The ideal night temperature for tuber formation is ~17 °C while temperature beyond 22 °C drastically reduces the tuber yield. Moreover, high temperature has several undesirable effects on the plant and tubers. Investigation of the genes involved in tuberization under heat stress can be helpful in the generation of heat-tolerant potato varieties. Five genes, including StSSH2 (succinic semialdehyde reductase isoform 2), StWTF (WRKY transcription factor), StUGT (UDP-glucosyltransferase), StBHP (Bel1 homeotic protein), and StFLTP (FLOWERING LOCUS T protein), involved in tuberization and heat stress in potato were investigated. The results of our microarray analysis suggested that these genes regulate and function as transcriptional factors, hormonal signaling, cellular homeostasis, and mobile tuberization signals under elevated temperature in contrasting KS (Kufri Surya) and KCM (Kufri Chandramukhi) potato cultivars. However, no detailed report is available which establishes functions of these genes in tuberization under heat stress. Thus, the present study was designed to validate the functions of these genes in tuber signaling and heat tolerance using virus-induced gene silencing (VIGS). Results indicated that VIGS transformed plants had a consequential reduction in StSSH2, StWTF, StUGT, StBHP, and StFLTP transcripts compared to the control plants. Phenotypic observations suggest an increase in plant senescence, reductions to both number and size of tubers, and a decrease in plant dry matter compared to the control plants. We also establish the potency of VIGS as a high-throughput technique for functional validation of genes.
- Subjects :
- Gene Expression Regulation, Plant genetics
Hot Temperature
Plant Proteins genetics
Plant Tubers growth & development
Plant Tubers virology
Solanum tuberosum growth & development
Solanum tuberosum virology
Temperature
Gene Silencing
Heat-Shock Response genetics
Plant Tubers genetics
Solanum tuberosum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1438-7948
- Volume :
- 21
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Functional & integrative genomics
- Publication Type :
- Academic Journal
- Accession number :
- 33611637
- Full Text :
- https://doi.org/10.1007/s10142-021-00771-2