A dynamic resazurin microassay allowing accurate quantification of cells and suitable for acid-forming bacteria.

Authors :: Labadie M
Randrianjatovo-Gbalou I
Zaidi-Ait-Salem M
Dossat-Létisse V
Fontagné-Faucher C
Marcato-Romain CE
Source :: Journal of microbiological methods [J Microbiol Methods] 2021 Apr; Vol. 183, pp. 106172. Date of Electronic Publication: 2021 Feb 18.
Publication Year :: 2021
Abstract: A resazurin micro-assay was developed to quantify acidifying bacteria. The resorufin fluorescent signal was measured over time and the determined time to reach the max slope (TMS) was plotted against CFU (colony forming unit) counts. This dynamic assay enabled to quantify nine lactic acid bacteria and a Bacillus licheniformis strain despite the increasing acidity of the medium.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)

Subjects :: Bacillus licheniformis chemistry
Bacillus licheniformis growth & development
Bacillus licheniformis metabolism
Culture Media chemistry
Culture Media metabolism
Fluorescence
Lactobacillales chemistry
Lactobacillales metabolism
Oxazines metabolism
Xanthenes metabolism
Acids metabolism
Bacteriological Techniques methods
Lactobacillales growth & development
Oxazines chemistry
Xanthenes chemistry

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