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TNFR2 expression is a hallmark of human memory B cells with suppressive function.
- Source :
-
European journal of immunology [Eur J Immunol] 2021 May; Vol. 51 (5), pp. 1195-1205. Date of Electronic Publication: 2021 Mar 08. - Publication Year :
- 2021
-
Abstract
- Tumor Necrosis Factor Receptor 2 (TNFR2) expression is increasingly being linked to tolerogenic immune reactions and cells with suppressor function including a subset of T-regulatory cells. B-regulatory cells play an important role in control of T-cell responses and inflammation. Recently, we described TNFR2 as a marker for IL-10-producing B cells, a hallmark of this cell subset. Here, we demonstrate that proliferation of T cells is reduced in the presence of TNFR2 positive human memory B cells generated with TLR9 ligand, while TNFR2- and TNFR2+CD27- B cells display costimulatory activity. Our data further reveal that IL-10 secretion is characteristic of IgM+ naïve and memory B cells but suppressive activity is not restricted to IL-10: (i) the inhibitory effect of TNFR2+ switched memory B cells was comparable to that exerted by TNFR2+ IgM+ memory B cells although IL-10 secretion levels in the cocultures were lower; (ii) supernatants from TNFR2+ memory B cells failed to suppress T-cell proliferation. Based on our findings, we propose that formation of Breg is a specific characteristic of human memory B cells undergoing terminal differentiation. Our data further corroborate that TNFR2 represents a viable marker for identification of memory B cells with regulatory function.<br /> (© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
- Subjects :
- B-Lymphocytes, Regulatory immunology
B-Lymphocytes, Regulatory metabolism
Case-Control Studies
Cell Communication immunology
Cell Differentiation immunology
Common Variable Immunodeficiency etiology
Common Variable Immunodeficiency metabolism
Cytokines metabolism
Humans
Interleukin-10 metabolism
Receptors, Tumor Necrosis Factor, Type II metabolism
T-Lymphocytes immunology
T-Lymphocytes metabolism
Toll-Like Receptor 9 metabolism
B-Lymphocytes immunology
B-Lymphocytes metabolism
Gene Expression Regulation
Immunologic Memory
Immunomodulation genetics
Lymphocyte Activation genetics
Lymphocyte Activation immunology
Receptors, Tumor Necrosis Factor, Type II genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 51
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33609401
- Full Text :
- https://doi.org/10.1002/eji.202048988