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Dysregulated transcriptional responses to SARS-CoV-2 in the periphery.
- Source :
-
Nature communications [Nat Commun] 2021 Feb 17; Vol. 12 (1), pp. 1079. Date of Electronic Publication: 2021 Feb 17. - Publication Year :
- 2021
-
Abstract
- SARS-CoV-2 infection has been shown to trigger a wide spectrum of immune responses and clinical manifestations in human hosts. Here, we sought to elucidate novel aspects of the host response to SARS-CoV-2 infection through RNA sequencing of peripheral blood samples from 46 subjects with COVID-19 and directly comparing them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS-CoV-2 infection triggers a powerful transcriptomic response in peripheral blood with conserved components that are heavily interferon-driven but also marked by indicators of early B-cell activation and antibody production. Interferon responses during SARS-CoV-2 infection demonstrate unique patterns of dysregulated expression compared to other infectious and healthy states. Heterogeneous activation of coagulation and fibrinolytic pathways are present in early COVID-19, as are IL1 and JAK/STAT signaling pathways, which persist into late disease. Classifiers based on differentially expressed genes accurately distinguished SARS-CoV-2 infection from other acute illnesses (auROC 0.95 [95% CI 0.92-0.98]). The transcriptome in peripheral blood reveals both diverse and conserved components of the immune response in COVID-19 and provides for potential biomarker-based approaches to diagnosis.
- Subjects :
- COVID-19 blood
COVID-19 virology
Cytokines genetics
Host-Pathogen Interactions
Humans
Influenza, Human genetics
Pneumonia, Bacterial genetics
SARS-CoV-2 physiology
Signal Transduction genetics
COVID-19 genetics
Gene Expression Profiling methods
Leukocytes, Mononuclear metabolism
Sequence Analysis, RNA methods
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 33597532
- Full Text :
- https://doi.org/10.1038/s41467-021-21289-y