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Disruption of mitochondrial complexes, cytotoxicity, and apoptosis results from Mancozeb exposure in transformed human colon cells.
- Source :
-
Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2021 May; Vol. 84, pp. 103614. Date of Electronic Publication: 2021 Feb 13. - Publication Year :
- 2021
-
Abstract
- Ethylene bisdithiocarbamate pesticides, including Mancozeb (MZ), are used as fungicides. Effects of MZ on apoptosis induction and mitochondrial activity of HT-29 colon cells were investigated. MZ exposed cells exhibited blebbing and cellular membrane disruption in scanning electron micrographs. Positive fluorescent staining with Annexin V at doses of 60-140 μM supports apoptosis as the mechanism of cell death. Activity of all electron transport chain complexes were evaluated. Mitochondrial Complex I activity was decreased in 100 μM treated cells. Mitochondrial Complex III activity was decreased in 60 and 100 μM MZ treated cells. Mitochondrial Complex II and Complex IV activities were decreased in cells treated with 60, 100, and 140 μM. Cells treated with 60 μM exhibited a decrease in Complex V enzyme activity. It is concluded that MZ exposure inhibits all mitochondrial complexes of HT-29 cells and that positive fluorescent microscopy and blebbing support previous studies of cell death via apoptosis.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Apoptosis drug effects
Cell Survival drug effects
Colon cytology
Electron Transport Chain Complex Proteins metabolism
HT29 Cells
Humans
Mitochondria metabolism
Electron Transport Chain Complex Proteins antagonists & inhibitors
Fungicides, Industrial toxicity
Maneb toxicity
Mitochondria drug effects
Zineb toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7077
- Volume :
- 84
- Database :
- MEDLINE
- Journal :
- Environmental toxicology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 33592315
- Full Text :
- https://doi.org/10.1016/j.etap.2021.103614