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Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection.
- Source :
-
PloS one [PLoS One] 2021 Feb 16; Vol. 16 (2), pp. e0246302. Date of Electronic Publication: 2021 Feb 16 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Background: Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated.<br />Methods: One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTM Express-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTM system (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control.<br />Results: No significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkit was the most efficient although the MMin-house presented, in average, lower Cts than the other two. In-house protocols allowed to save between 350€ and 400€ for every 96 extracted samples compared to the commercial kit.<br />Conclusion: The protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Automation, Laboratory economics
Automation, Laboratory standards
COVID-19 Nucleic Acid Testing economics
COVID-19 Nucleic Acid Testing standards
Costs and Cost Analysis
Humans
RNA, Viral chemistry
RNA, Viral genetics
Reagent Kits, Diagnostic economics
Reagent Kits, Diagnostic standards
Sensitivity and Specificity
Automation, Laboratory methods
COVID-19 Nucleic Acid Testing methods
RNA, Viral standards
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 33591986
- Full Text :
- https://doi.org/10.1371/journal.pone.0246302