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Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles.
- Source :
-
Scientific reports [Sci Rep] 2021 Feb 15; Vol. 11 (1), pp. 3849. Date of Electronic Publication: 2021 Feb 15. - Publication Year :
- 2021
-
Abstract
- Even though application of nanoparticles in medicine seems to provide unique solutions for drug delivery and diagnosis diseases, understanding interactions between nanoscale materials and biological systems is imperative. Therefore, this study determined the effect of different types of nanoparticles (NPs) on human endothelial cells and examined the types of toxicity responses they can induce. Four different types of NPs were tested (PLA/MMT/TRASTUZUMAB, PLA/EDTMP, PLGA/MDP, and Pluronic F127 MICELLES), representing three putative areas of application: anticancer therapy, scintigraphy, and cosmetology. The experiments were performed on immortalized human umbilical vein endothelial cells (HUVEC-STs). Light contrast phase microscopy as well as cell viability assays showed that only Pluronic F127 MICELLES decreased the number of HUVEC-STs in contrast to PLA/MMT/TRASTUZUMAB, PLA/EDTMP, and PLGA/MDP NPs, which altered cell morphology, but not their confluency. The tested NPs induced not only DNA strand-breaks and alkali-labile sites, but also internucleosomal DNA fragmentation, visualized as a DNA ladder pattern typical of apoptosis. Moreover, generation of free radicals and subsequent mitochondrial membrane potential collapse showed the significance of free radical production during interactions between NPs and endothelial cells. High concentrations of NPs had different degrees of toxicity in human endothelial cells and affected cell proliferation, redox homeostasis, and triggered mitochondrial dysfunction.
- Subjects :
- Apoptosis drug effects
Cell Line
Cell Survival
Cells, Cultured
Chemical Phenomena
DNA Damage
DNA Fragmentation
Drug Carriers
Humans
Mitochondria drug effects
Mitochondria genetics
Mitochondria metabolism
Oxidative Stress
Particle Size
Biomarkers
Homeostasis drug effects
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells metabolism
Nanoparticles adverse effects
Nanoparticles chemistry
Nanoparticles ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33589697
- Full Text :
- https://doi.org/10.1038/s41598-021-83291-0