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Proteome-wide and matrisome-specific alterations during human pancreas development and maturation.
- Source :
-
Nature communications [Nat Commun] 2021 Feb 15; Vol. 12 (1), pp. 1020. Date of Electronic Publication: 2021 Feb 15. - Publication Year :
- 2021
-
Abstract
- The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.
- Subjects :
- Adolescent
Adult
Child
Child, Preschool
Chromatography, Liquid
Extracellular Matrix genetics
Extracellular Matrix metabolism
Extracellular Matrix Proteins classification
Extracellular Matrix Proteins metabolism
Female
Fetus
Fluorescent Antibody Technique
Gene Ontology
Humans
Male
Middle Aged
Molecular Sequence Annotation
Organogenesis genetics
Pancreas growth & development
Proteome classification
Proteome metabolism
Proteomics methods
Tandem Mass Spectrometry
Extracellular Matrix Proteins genetics
Gene Expression Regulation, Developmental
Pancreas metabolism
Proteome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 33589611
- Full Text :
- https://doi.org/10.1038/s41467-021-21261-w