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Enhanced Extracellular Production of Is PETase in Escherichia coli via Engineering of the pelB Signal Peptide.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2021 Feb 24; Vol. 69 (7), pp. 2245-2252. Date of Electronic Publication: 2021 Feb 12. - Publication Year :
- 2021
-
Abstract
- Poly(ethylene terephthalate) (PET) is one of the most commonly used plastics worldwide and its accumulation in the environment is a global problem. PETase from Ideonella sakaiensis 201-F6 was reported to exhibit higher hydrolytic activity and specificity for PET than other enzymes at ambient temperature. Enzymatic degradation of PET using PETase provides an attractive approach for plastic degradation and recycling. In this work, extracellular PETase was achieved by Escherichia coli BL21 using a Sec-dependent translocation signal peptide, pelB, for secretion. Furthermore, engineering of the pelB through random mutagenesis and screening was performed to improve the secretion efficiency of PETase. Evolved pelB enabled higher PETase secretion by up to 1.7-fold. The improved secretion of PETase led to more efficient hydrolysis of the PET model compound, bis (2-hydroxyethyl) terephthalic acid (BHET), PET powder, and PET film. Our study presents the first example of the increasing secretion of PETase by an engineered signal peptide, providing a promising approach to obtain extracellular PETase for efficient enzymatic degradation of PET.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 69
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 33576230
- Full Text :
- https://doi.org/10.1021/acs.jafc.0c07469