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Memory T helper cells identify patients with nickel, cobalt, and chromium metal allergy.
- Source :
-
Contact dermatitis [Contact Dermatitis] 2021 Jul; Vol. 85 (1), pp. 7-16. Date of Electronic Publication: 2021 Mar 22. - Publication Year :
- 2021
-
Abstract
- Background: Patch testing is the gold standard for identifying culprit allergens in allergic contact dermatitis; however, it is laborious and positive reactions are difficult to quantitate. Development of complementary in vitro tests is, therefore, of great importance.<br />Objectives: This study aimed to improve the in vitro lymphocyte proliferation test (LPT) to detect allergic responses to nickel (Ni), cobalt (Co), and chromium (Cr).<br />Methods: Twenty-one metal allergic patients with a positive patch test to Ni (n=16), Co (n=8), and Cr (n=3) and 13 controls were included. All were tested by a flow cytometric LPT.<br />Results: Metal-reactive cells were identified as T helper (Th) cells with high expression of the memory marker CD45RO. Skin-homing (cutaneous lymphocyte-associated antigen positive [CLA+]) Ni-reactive memory Th (Th <subscript>mem</subscript> <superscript>hi</superscript> ) cells identified individuals with a positive patch test for Ni with 100% sensitivity (95% confidence interval [CI] 81%-100%) and 92% specificity (95% CI 67%-100%). Moreover, Co-specific Th <subscript>mem</subscript> <superscript>hi</superscript> cells expressing CCR6 identified patients with a positive patch test for Co with 63% sensitivity (95% CI 31%-86%) and 100% specificity (95% CI 77%-100%). In Cr allergic individuals, Cr-reactive Th <subscript>mem</subscript> <superscript>hi</superscript> cells tended to increased CLA and CCR6 expression.<br />Conclusion: Metal-reactive Th cells with high expression of CD45RO and coexpression of CLA and CCR6 improved the LPT, making it an attractive supplement to the patch test.<br /> (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Details
- Language :
- English
- ISSN :
- 1600-0536
- Volume :
- 85
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Contact dermatitis
- Publication Type :
- Academic Journal
- Accession number :
- 33576045
- Full Text :
- https://doi.org/10.1111/cod.13809