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Mapping circular RNA structures in living cells by SHAPE-MaP.
- Source :
-
Methods (San Diego, Calif.) [Methods] 2021 Dec; Vol. 196, pp. 47-55. Date of Electronic Publication: 2021 Feb 09. - Publication Year :
- 2021
-
Abstract
- Circular RNAs are produced from back-splicing of exons of precursor mRNAs (pre-mRNAs). The sequences of exons in circular RNAs are identical to their linear cognate mRNAs, but the circular format may confer constraints on their folding and conformation, leading to potentially different functions from their linear RNA cognates. Here, we describe experimental and computational steps that optimize the selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) to probe circular RNA secondary structure at single-nucleotide resolution in living cells.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1095-9130
- Volume :
- 196
- Database :
- MEDLINE
- Journal :
- Methods (San Diego, Calif.)
- Publication Type :
- Academic Journal
- Accession number :
- 33571668
- Full Text :
- https://doi.org/10.1016/j.ymeth.2021.01.011