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A colorimetric paper-based ATONP-ALP nanobiosensor for selective detection of Cd 2+ ions in clams and mussels.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Mar; Vol. 413 (6), pp. 1715-1727. Date of Electronic Publication: 2021 Feb 10. - Publication Year :
- 2021
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Abstract
- A colorimetric paper-based enzyme-coupled antimony tin oxide nanoparticle (ATONP) nanobiosensor for selective detection of Cd <superscript>2+</superscript> ions in clams and mussels is presented. Alkaline phosphatase (ALP) was immobilized on ATONPs via 16-phosphonohexadecanoic acid (16-PHA) to develop ATONP-ALP nanobiosensor. The biosensor was characterized using XPS, Raman spectroscopy, SEM, and EDX. ATONP-ALP nanobiosensor exhibited high selectivity towards detection of Cd <superscript>2+</superscript> ion with a LOD 0.006 μg L <superscript>-1</superscript> and linear range of detection 0.005-1 μg L <superscript>-1</superscript> . The developed biosensor was further integrated into a low-cost paper-based format. A visual color change was obtained for Cd <superscript>2+</superscript> ion in the range 0.1-10 μg L <superscript>-1</superscript> . The developed biosensor was successfully demonstrated for the analysis of Cd <superscript>2+</superscript> ions in clams with recoveries 101-104%. The ATONP-ALP nanobiosensor was validated using mussel tissue (BCR-668) and the conventional ICP-OES and ICP-MS techniques.
- Subjects :
- Alkaline Phosphatase chemistry
Animals
Bivalvia
Calibration
Food Analysis methods
Food Contamination analysis
Kinetics
Limit of Detection
Microscopy, Electron, Scanning
Palmitic Acid chemistry
Paper
Reproducibility of Results
Seafood
Spectrum Analysis, Raman
Biosensing Techniques
Cadmium chemistry
Colorimetry methods
Ions
Nanotechnology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 413
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 33564927
- Full Text :
- https://doi.org/10.1007/s00216-020-03140-3