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NEK5 interacts with LonP1 and its kinase activity is essential for the regulation of mitochondrial functions and mtDNA maintenance.
- Source :
-
FEBS open bio [FEBS Open Bio] 2021 Mar; Vol. 11 (3), pp. 546-563. Date of Electronic Publication: 2021 Feb 24. - Publication Year :
- 2021
-
Abstract
- Little is known about Nima-related kinase (NEKs), a widely conserved family of kinases that have key roles in cell-cycle progression. Nevertheless, it is now clear that multiple NEK family members act in networks, not only to regulate specific events of mitosis, but also to regulate metabolic events independently of the cell cycle. NEK5 was shown to act in centrosome disjunction, caspase-3 regulation, myogenesis, and mitochondrial respiration. Here, we demonstrate that NEK5 interacts with LonP1, an AAA+ mitochondrial protease implicated in protein quality control and mtDNA remodeling, within the mitochondria and it might be involved in the LonP1-TFAM signaling module. Moreover, we demonstrate that NEK5 kinase activity is required for maintaining mitochondrial mass and functionality and mtDNA integrity after oxidative damage. Taken together, these results show a new role of NEK5 in the regulation of mitochondrial homeostasis and mtDNA maintenance, possibly due to its interaction with key mitochondrial proteins, such as LonP1.<br /> (© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Subjects :
- Cell Line
DNA Copy Number Variations
Gene Expression Regulation
HEK293 Cells
Humans
Mitochondria genetics
NIMA-Related Kinases genetics
Oxidative Stress
Protein Interaction Maps
ATP-Dependent Proteases metabolism
DNA, Mitochondrial genetics
Mitochondria metabolism
Mitochondrial Proteins metabolism
NIMA-Related Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-5463
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS open bio
- Publication Type :
- Academic Journal
- Accession number :
- 33547867
- Full Text :
- https://doi.org/10.1002/2211-5463.13108