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Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds.
- Source :
-
Scientific reports [Sci Rep] 2021 Feb 04; Vol. 11 (1), pp. 3059. Date of Electronic Publication: 2021 Feb 04. - Publication Year :
- 2021
-
Abstract
- Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being developed to tackle these pressing issues require effective in-vitro culture platforms. Electrospun scaffolds provide bio-mimetic structures upon which cells are cultured to regulate function in-vitro. This study aims to shed light on the effects of electrospun PCL morphology on the culture of an immortalised hepatic cell line and mouse primary hepatocytes. Each cell type was cultured on large 4-5 µm fibres and small 1-2 µm fibres with random, aligned and highly porous cryogenically spun configurations. Cell attachment, proliferation, morphology and functional protein and gene expression was analysed. Results show that fibre morphology has a measurable influence on cellular morphology and function, with the alteration of key functional markers such as CYP1A2 expression.
- Subjects :
- Animals
Biomimetics
Cell Proliferation genetics
Gene Expression Regulation
Hep G2 Cells
Hepatocytes metabolism
Hepatocytes pathology
Humans
Liver growth & development
Liver pathology
Liver Diseases genetics
Liver Diseases pathology
Mice
Tissue Engineering methods
Cytochrome P-450 CYP1A2 genetics
Liver metabolism
Liver Diseases therapy
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33542251
- Full Text :
- https://doi.org/10.1038/s41598-021-81761-z