Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.

In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application. Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe ₃ O ₄ ) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics. Well-characterized Anti-Abs _CA19-9 /CS-MWCNT-Fe ₃ O ₄ immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg ^-1 cm ^-1 ), a detection limit of 0.163 pg mL ^-1 , detection range from 1.0 pg mL ^-1 to 100 ng mL ^-1 . Our fabricated electrochemical Abs _CA19-9 /CS-MWCNT-Fe ₃ O ₄ immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy. To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). The results of the studies projected Abs _CA19-9 /CS-MWCNT-Fe ₃ O ₄ electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA. We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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