Unveiling the effect of interacting forecasted abiotic factors on growth and aflatoxin B 1 production kinetics by Aspergillus flavus.

The aim was to decipher the temporal impact of key interacting climate change (CC) abiotic factors of temperature (30 vs 37 °C), water activity (a _w ; 0.985 vs 0.930) and CO ₂ exposure (400 vs 1000 ppm) on (a) growth of Aspergillus flavus and effects on (b) gene expression of a structural (aflD) and key regulatory gene (aflR) involved in aflatoxin B ₁ (AFB ₁ ) biosynthesis and (c) AFB ₁ production on a yeast extract sucrose medium over a period of 10 days. A. flavus grew and produced AFB ₁ very early with toxin detected after only 48 h. Both growth and toxin production were significantly impacted by the interacting abiotic factors. The relative expression of the aflD gene was significantly influenced by temperature; aflR gene expression was mainly modulated by time. However, no clear relationship was observed for both genes with AFB ₁ production over the experimental time frame. The optimum temperature for AFB ₁ production was 30 °C. Maximum AFB ₁ production occurred between days 4-8. Exposure to higher CO ₂  conditions simulating forecasted CC conditions resulted in the amount of AFB ₁  produced in elevated temperature (37 °C) being higher than with the optimum temperature (30 °C) showing a potential for increased risk for human/animal health due to higher accumulation of this toxin.
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