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Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2021 Feb 25; Vol. 57 (16), pp. 2073-2076. Date of Electronic Publication: 2021 Jan 28. - Publication Year :
- 2021
-
Abstract
- We demonstrate the development of a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification. This biosensor possesses the characteristics of good selectivity and high sensitivity with a detection limit of 7.3 × 10 <superscript>-17</superscript> M. It can distinguish as low as 0.01% methylation level, and can analyze genomic DNA methylation even in a single cancer cell.
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 57
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 33507186
- Full Text :
- https://doi.org/10.1039/d0cc08306b