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Distinct antibody repertoires against endemic human coronaviruses in children and adults.
- Source :
-
JCI insight [JCI Insight] 2021 Feb 22; Vol. 6 (4). Date of Electronic Publication: 2021 Feb 22. - Publication Year :
- 2021
-
Abstract
- Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these "common cold" viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires in 231 children and 1168 adults using phage immunoprecipitation sequencing. Seroprevalence of antibodies against endemic HCoVs ranged between approximately 4% and 27% depending on the species and cohort. We identified at least 136 novel linear B cell epitopes. Antibody repertoires against endemic HCoVs were qualitatively different between children and adults in that anti-HCoV IgG specificities more frequently found among children targeted functionally important and structurally conserved regions of the spike, nucleocapsid, and matrix proteins. Moreover, antibody specificities targeting the highly conserved fusion peptide region and S2' cleavage site of the spike protein were broadly cross-reactive with peptides of epidemic human and nonhuman coronaviruses. In contrast, an acidic tandem repeat in the N-terminal region of the Nsp3 subdomain of the HCoV-HKU1 polyprotein was the predominant target of antibody responses in adult donors. Our findings shed light on the dominant species-specific and pan-CoV target sites of human antibody responses to coronavirus infection, thereby providing important insights for the development of prophylactic or therapeutic monoclonal antibodies and vaccine design.
- Subjects :
- Adult
Antibodies, Viral blood
Antibodies, Viral immunology
Antibody Specificity
Antigens, Viral blood
Antigens, Viral immunology
Child
Child, Preschool
Common Cold blood
Common Cold epidemiology
Common Cold immunology
Coronavirus isolation & purification
Coronavirus Infections blood
Coronavirus Infections epidemiology
Coronavirus Infections virology
Cross Reactions
Epitopes, B-Lymphocyte blood
Epitopes, B-Lymphocyte immunology
Female
Humans
Male
Middle Aged
Protein Domains immunology
Retrospective Studies
Seroepidemiologic Studies
Viral Proteins immunology
Antibodies, Viral isolation & purification
Common Cold virology
Coronavirus immunology
Coronavirus Infections immunology
Endemic Diseases
Subjects
Details
- Language :
- English
- ISSN :
- 2379-3708
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- JCI insight
- Publication Type :
- Academic Journal
- Accession number :
- 33497357
- Full Text :
- https://doi.org/10.1172/jci.insight.144499