Serosurvey of Coxiella burnetii infection in dairy goat herds in Ontario. A comparison of two methods of enzyme-linked immunosorbent assay.

Authors :: Lang GH
Source :: Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 1988 Jan; Vol. 52 (1), pp. 37-41.
Publication Year :: 1988
Abstract: Two technical variations of enzyme-linked immunosorbent assay for the detection of antibodies to Coxiella burnetii were compared in this serosurvey on 20 Ontario dairy goat herds. Both a trichloracetic acid extract and a coctoantigen of purified coxiellas were used to sensitize the microtitration plates. Technical differences related to coating pH, serum dilutions tested and interpretation of results. Results agreed in 98.6% of sera examined, the differing sera were in the low titer borderline range. Only 20% of the herds had seroreactors.

Subjects :: Animals
Antigens, Bacterial immunology
Female
Hydrogen-Ion Concentration
Ontario
Predictive Value of Tests
Q Fever epidemiology
Antibodies, Bacterial analysis
Coxiella immunology
Enzyme-Linked Immunosorbent Assay
Goats
Q Fever veterinary

Language :: English
ISSN :: 0830-9000
Volume :: 52
Issue :: 1
Database :: MEDLINE
Journal :: Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
Publication Type :: Academic Journal
Accession number :: 3349400

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