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Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples.

Authors :
Kumar A
Ghazi MGU
Singh B
Hussain SA
Bhatt D
Gupta SK
Source :
Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour] 2017 Feb 06; Vol. 2 (1), pp. 64-66. Date of Electronic Publication: 2017 Feb 06.
Publication Year :
2017

Abstract

We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301-1599 bp DNA fragments in various combinations. The method described may assist in the sequencing of either complete gene from moderate to good quality DNA or shorter fragment from degraded DNA.<br />Competing Interests: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.<br /> (© 2017 Wildlife Institute of India. Published by Informa UK Limited, trading as Taylor & Francis Group.)

Details

Language :
English
ISSN :
2380-2359
Volume :
2
Issue :
1
Database :
MEDLINE
Journal :
Mitochondrial DNA. Part B, Resources
Publication Type :
Academic Journal
Accession number :
33490438
Full Text :
https://doi.org/10.1080/23802359.2016.1247672