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The wheat growth-promoting traits of Ochrobactrum and Pantoea species, responsible for solubilization of different P sources, are ensured by genes encoding enzymes of multiple P-releasing pathways.
- Source :
-
Microbiological research [Microbiol Res] 2021 May; Vol. 246, pp. 126703. Date of Electronic Publication: 2021 Jan 15. - Publication Year :
- 2021
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Abstract
- Production and release of organic acids and phosphatase enzymes by microbes are important for inorganic and organic phosphorus cycling in soil. The presence of microorganisms with corresponding traits in the plant rhizosphere lead to improved plant P uptake and ultimately growth promotion. We studied the potential of two rhizosphere-competent strains, Pantoea sp. MR1 and Ochrobactrum sp. SSR, for solubilization of different organic and inorganic P sources in vitro. In a pot experiment we further revealed the impact of the two strains on wheat seedling performance in soil amended with either phytate, rock phosphate or K <subscript>2</subscript> HPO <subscript>4</subscript> as solely P source. To directly link P-solubilizing activity to the strain-specific genetic potential, we designed novel primers for glucose dehydrogenase (gcd), phosphatase (pho) and phytase (phy) genes, which are related to the organic and inorganic P solubilization potential. Quantitative tracing of these functional genes in the inoculated soils of the conducted pot experiment further allowed to compare strain abundances in the soil in dependency on the present P source. We observed strain- and P source-dependent patterns of the P solubilization in vitro as well as in the pot experiment, whereby P release, particularly from phytate, was linked to the strain abundance. We further revealed that the activity of microbial phosphatases is determined by the interplay between functional gene abundance, available soil P, and substrate availability. Moreover, positive impacts of microbial seed inoculation on wheat root architecture and aboveground growth parameters were observed. Our results suggest that screening for rhizosphere-competent strains with gcd, pho and phy genes may help to identify new microbial taxa that are able to solubilize and mineralize inorganic as well as organic bound P. Subsequently, the targeted use of corresponding strains may improve P availability in agricultural soils and consequently reduce fertilizer application.<br /> (Copyright © 2021 Elsevier GmbH. All rights reserved.)
- Subjects :
- 6-Phytase genetics
Bacterial Proteins genetics
Glucose 1-Dehydrogenase genetics
Ochrobactrum enzymology
Pantoea enzymology
Phosphates metabolism
Phosphoric Monoester Hydrolases genetics
Phylogeny
Phytic Acid metabolism
Plant Roots microbiology
Rhizosphere
Seedlings growth & development
Seedlings metabolism
Soil chemistry
Soil Microbiology
Triticum metabolism
Ochrobactrum genetics
Pantoea genetics
Phosphorus metabolism
Triticum growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0623
- Volume :
- 246
- Database :
- MEDLINE
- Journal :
- Microbiological research
- Publication Type :
- Academic Journal
- Accession number :
- 33482437
- Full Text :
- https://doi.org/10.1016/j.micres.2021.126703