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Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2238, pp. 135-143. - Publication Year :
- 2021
-
Abstract
- The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.
- Subjects :
- Cytidine Deaminase genetics
Gene Transfer Techniques
Genetic Vectors genetics
Genome, Plant
Oryza genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified growth & development
Seeds genetics
Transformation, Genetic
Transgenes physiology
CRISPR-Cas Systems
Cytidine Deaminase antagonists & inhibitors
Gene Editing
Oryza growth & development
Plant Breeding
Polymorphism, Single Nucleotide
Seeds growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2238
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33471329
- Full Text :
- https://doi.org/10.1007/978-1-0716-1068-8_9