Back to Search
Start Over
Properties of component X of rat heart pyruvate dehydrogenase complex.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1988 Jan 29; Vol. 150 (2), pp. 816-21. - Publication Year :
- 1988
-
Abstract
- Pyruvate dehydrogenase complex was purified from rat heart. A new component(mol.wt; 52,000) was found in the purified complex in addition to well known three component enzymes. This component(referred to as component X) was acetylated with [2-14C] pyruvate in the absence of CoA as well as lipoate acetyltransferase. The anti-lipoate acetyltransferase antibody reacted with component X and lipoate acetyltransferase, suggesting that component X shows homology with lipoate acetyltransferase in protein structure. cDNA for lipoate acetyltransferase was isolated from rat liver cDNA library in lambda gt 11. cDNA for lipoate acetyltransferase recognized two kinds of mRNAs of 3.5 Kb and 2.5 Kb.
- Subjects :
- Acetyltransferases genetics
Acetyltransferases immunology
Acetyltransferases metabolism
Animals
Antibodies
Base Composition
Cloning, Molecular
Dihydrolipoyllysine-Residue Acetyltransferase
Molecular Weight
Protein Biosynthesis
Pyruvate Dehydrogenase Complex immunology
Pyruvate Dehydrogenase Complex metabolism
Rats
Liver enzymology
Myocardium enzymology
Pyruvate Dehydrogenase Complex genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 150
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 3342049
- Full Text :
- https://doi.org/10.1016/0006-291x(88)90464-0