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Insight into the Mechanism of Action and Peptide-Membrane Interactions of Aib-Rich Peptides: Multitechnique Experimental and Theoretical Analysis.

Authors :
Lizio MG
Campana M
De Poli M
Jefferies DF
Cullen W
Andrushchenko V
Chmel NP
Bouř P
Khalid S
Clayden J
Blanch E
Rodger A
Webb SJ
Source :
Chembiochem : a European journal of chemical biology [Chembiochem] 2021 May 04; Vol. 22 (9), pp. 1656-1667. Date of Electronic Publication: 2021 Feb 24.
Publication Year :
2021

Abstract

The increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib-rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane-embedded peptides. The protocols presented in this paper open new ground by showing how chiro-optical spectroscopies can throw light on the mechanism of action of AMPs.<br /> (© 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH.)

Details

Language :
English
ISSN :
1439-7633
Volume :
22
Issue :
9
Database :
MEDLINE
Journal :
Chembiochem : a European journal of chemical biology
Publication Type :
Academic Journal
Accession number :
33411956
Full Text :
https://doi.org/10.1002/cbic.202000834