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The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia.
- Source :
-
NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2021 Jan 05; Vol. 7 (1), pp. 1. Date of Electronic Publication: 2021 Jan 05. - Publication Year :
- 2021
-
Abstract
- Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous peoples of Malaysia. A total of 130 participants were recruited from two sites in the north-eastern state of Terengganu in Peninsular Malaysia. Using 16S rRNA sequencing, the nasal microbiome was significantly more diverse in those aged 5-17 years compared to 50+ years (p = 0.023) and clustered by age (PERMANOVA analysis of the Bray-Curtis distance, p = 0.001). Hierarchical clustering of Bray-Curtis dissimilarity scores revealed six microbiome clusters. The largest cluster (n = 28; 35.4%) had a marked abundance of Corynebacterium. In the oral microbiomes Streptococcus, Neisseria and Haemophilus were dominant. Using conventional microbiology, high levels of Staphylococcus aureus carriage were observed, particularly in the 18-65 age group (n = 17/36; 47.2% 95% CI: 30.9-63.5). The highest carriage of pneumococci was in the <5 and 5 to 17 year olds, with 57.1% (4/7) and 49.2% (30/61), respectively. Sixteen pneumococcal serotypes were identified, the most common being the nonvaccine-type 23A (14.6%) and the vaccine-type 6B (9.8%). The prevalence of pneumococcal serotypes covered by pneumococcal conjugate vaccines support introduction into a Malaysian national immunisation schedule. In addition, the dominance of Corynebacterium in the airway microbiomes is intriguing given their role as a potentially protective commensal with respect to acute infection and respiratory health.
- Subjects :
- Adolescent
Adult
Aged
Anti-Bacterial Agents pharmacology
Bacteria classification
Bacteria drug effects
Bacteria genetics
Bacteria isolation & purification
Child
Child, Preschool
Cluster Analysis
Drug Resistance, Bacterial
Female
Humans
Malaysia
Male
Middle Aged
Mouth microbiology
Nasal Cavity microbiology
Prevalence
RNA, Ribosomal, 16S genetics
Serogroup
Streptococcus pneumoniae classification
Streptococcus pneumoniae drug effects
Streptococcus pneumoniae genetics
Streptococcus pneumoniae isolation & purification
Young Adult
Indigenous Peoples statistics & numerical data
Microbiota
Respiratory System microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2055-5008
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- NPJ biofilms and microbiomes
- Publication Type :
- Academic Journal
- Accession number :
- 33402693
- Full Text :
- https://doi.org/10.1038/s41522-020-00173-5