Back to Search
Start Over
HPLC Determination of Imidazoles with Variant Anti-Infective Activity in Their Dosage Forms and Human Plasma.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2020 Dec 30; Vol. 26 (1). Date of Electronic Publication: 2020 Dec 30. - Publication Year :
- 2020
-
Abstract
- A suitable HPLC method has been selected and validated for rapid simultaneous separation and determination of four imidazole anti-infective drugs, secnidazole, omeprazole, albendazole, and fenbendazole, in their final dosage forms, in addition to human plasma within 5 min. The method suitability was derived from the superiority of using the environmentally benign solvent, methanol over acetonitrile as a mobile phase component in respect of safety issues and migration times. Separation of the four anti-infective drugs was performed on a Thermo Scientific <superscript>®</superscript> BDS Hypersil C <subscript>8</subscript> column (5 µm, 2.50 × 4.60 mm) using a mobile phase consist of MeOH: 0.025 M KH <subscript>2</subscript> PO <subscript>4</subscript> (70:30, v/v ) adjusted to pH 3.20 with ortho-phosphoric acid at room temperature. The flow rate was 1.00 mL/min and maximum absorption was measured with UV detector set at 300 nm. Limits of detection were reported to be 0.41, 0.13, 0.18, and 0.15 µg/mL for secnidazole, omeprazole, albendazole, and fenbendazole, respectively, showing a high degree of the method sensitivity. The method of analysis was validated according to Food and Drug Administration (FDA)guidelines for the determination of the drugs, either in their dosage forms with highly precise recoveries, or clinically in human plasma, especially regarding pharmacokinetic and bioequivalence studies.
- Subjects :
- Albendazole analysis
Albendazole blood
Calibration
Chemistry Techniques, Analytical
Dosage Forms
Fenbendazole analysis
Fenbendazole blood
Humans
Hydrogen-Ion Concentration
Imidazoles blood
Metronidazole analogs & derivatives
Metronidazole analysis
Metronidazole blood
Omeprazole analysis
Omeprazole blood
Patient Safety
Reproducibility of Results
Solvents
Temperature
Ultraviolet Rays
United States
United States Food and Drug Administration
Anti-Infective Agents chemistry
Chemistry, Pharmaceutical methods
Chromatography, High Pressure Liquid methods
Imidazoles analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 33396585
- Full Text :
- https://doi.org/10.3390/molecules26010129