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Octominin: An antibacterial and anti-biofilm peptide for controlling the multidrug resistance and pathogenic Streptococcus parauberis.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2021 Mar; Vol. 110, pp. 23-34. Date of Electronic Publication: 2020 Dec 27. - Publication Year :
- 2021
-
Abstract
- Streptococcus parauberis is a pathogenic gram-positive bacterium that causes streptococcosis infection in fish. Since S. parauberis is becoming resistant to multiple antibiotics, the development of alternatives, such as antimicrobial peptides, has gained great attention. Octominin, derived from the defense protein of Octopus minor, showed a significant antimicrobial activity against multidrug resistance S. parauberis, with a minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of 50 and 100 μg/mL, respectively. Furthermore, time-kill kinetics, agar diffusion, and bacterial viability assays confirmed the concentration-dependent antibacterial activity of Octominin against S. parauberis. Field emission scanning electron microscopy analysis showed morphological and ultra-structural changes in S. parauberis upon Octominin treatment. Moreover, Octominin treatment demonstrated changes in membrane permeability, induced reactive oxygen species (ROS), and its binding ability to genomic DNA, suggesting its strong bactericidal activity with multiple modes of action. We confirmed the inhibition of biofilm formation and the eradication of existing biofilms in a concentration-dependent manner. Additionally, Octominin on S. parauberis at transcriptional level exhibited downregulation of membrane formation (pgsA and cds1), DNA repairing (recF), biofilm formation (pgaB and epsF) genes, while upregulation of ROS detoxification (sodA) and DNA protecting (ahpF) related genes. An in vivo study confirmed a significantly (P < 0.05) higher relative percentage survival in Octominin-treated larval zebrafish exposed to S. parauberis (93.3%) compared to the control group (20.0%). Collectively, our results confirm that Octominin could be a potential antibacterial and anti-biofilm agent against S. parauberis.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Drug Resistance, Multiple, Bacterial
Fish Diseases prevention & control
Microbial Sensitivity Tests veterinary
Microscopy, Electron, Scanning
Streptococcal Infections prevention & control
Streptococcal Infections veterinary
Streptococcus physiology
Streptococcus ultrastructure
Anti-Bacterial Agents pharmacology
Antimicrobial Cationic Peptides pharmacology
Biofilms drug effects
Microbial Viability drug effects
Peptide Fragments pharmacology
Streptococcus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 110
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33378697
- Full Text :
- https://doi.org/10.1016/j.fsi.2020.12.017