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The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans.
- Source :
-
Molecular cell [Mol Cell] 2021 Feb 04; Vol. 81 (3), pp. 546-557.e5. Date of Electronic Publication: 2020 Dec 29. - Publication Year :
- 2021
-
Abstract
- Eukaryotic cells regulate 5'-triphosphorylated RNAs (ppp-RNAs) to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR-1 (phosphatase that interacts with RNA and ribonucleoprotein particle 1) family of RNA polyphosphatases remove both the β and γ phosphates from ppp-RNAs. Here, we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RNA-dependent RNA polymerases (RdRPs) and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and Dicer-independent Argonaute pathways and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Animals, Genetically Modified
Caenorhabditis elegans embryology
Caenorhabditis elegans genetics
Caenorhabditis elegans Proteins genetics
Gene Expression Regulation, Developmental
Phosphoric Monoester Hydrolases genetics
Phosphorylation
RNA genetics
RNA Caps
RNA-Dependent RNA Polymerase genetics
RNA-Dependent RNA Polymerase metabolism
Ribonuclease III genetics
Ribonuclease III metabolism
Spermatogenesis
Substrate Specificity
Caenorhabditis elegans enzymology
Caenorhabditis elegans Proteins metabolism
Phosphoric Monoester Hydrolases metabolism
RNA metabolism
RNA Processing, Post-Transcriptional
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 81
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 33378643
- Full Text :
- https://doi.org/10.1016/j.molcel.2020.12.004