Back to Search
Start Over
A Protocol for Studying HIV-1 Envelope Glycoprotein Function.
- Source :
-
STAR protocols [STAR Protoc] 2020 Oct 14; Vol. 1 (3), pp. 100133. Date of Electronic Publication: 2020 Oct 14 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- HIV-1 envelope glycoproteins (Envs) bind to CD4 receptor and CCR5/CXCR4 coreceptor and mediate viral entry (Feng et al., 1996; Herschhorn et al., 2016, 2017; Kwong et al., 1998). HIV-1 Envs are the sole target of neutralizing antibodies and a main focus of vaccine development (Flemming et al., 2018). Here, we provide a step-by-step protocol to measure Env sensitivity to ligands, cold, and small molecules, as well as to study viral infectivity and to dissect parameters affecting HIV-1 Env function. For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).<br />Competing Interests: The authors declare no competing interests.<br /> (© 2020 The Author(s).)
- Subjects :
- Antibodies, Neutralizing immunology
CD4 Antigens metabolism
Genes, env genetics
Glycoproteins drug effects
Glycoproteins isolation & purification
Glycoproteins physiology
HIV Antibodies immunology
HIV-1 genetics
HIV-1 metabolism
Humans
Ligands
Receptors, CCR5 metabolism
Virus Internalization
env Gene Products, Human Immunodeficiency Virus metabolism
Drug Evaluation, Preclinical methods
env Gene Products, Human Immunodeficiency Virus drug effects
env Gene Products, Human Immunodeficiency Virus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 1
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 33377027
- Full Text :
- https://doi.org/10.1016/j.xpro.2020.100133