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Safety and bioactivity assessment of aqueous extract of Thai Henna ( Lawsonia inermis Linn.) Leaf.
- Source :
-
Journal of toxicology and environmental health. Part A [J Toxicol Environ Health A] 2021 Apr 03; Vol. 84 (7), pp. 298-312. Date of Electronic Publication: 2020 Dec 29. - Publication Year :
- 2021
-
Abstract
- The worldwide demand for a natural dye by the cosmetic and food industry has recently gained interest. To provide scientific data supporting the usage of Thai henna leaf as a natural colorant, the phytochemical constituents, safety, and bioactivity of aqueous extract of the henna leaf by autoclave (HAE) and hot water (HHE) were determined. HAE contained a higher amount of total phenolic and flavonoid contents than HHE. The major constituents in both extracts were ferulic acid, gallic acid, and luteolin. The extracts displayed no marked mutagenic activity both in vitro and in vivo mammalian-like biotransformation. HAE and HHE also exhibited non-cytotoxicity to human immortalized keratinocyte cells (HaCaT), peripheral blood mononuclear cells (PBMCs), and murine macrophage RAW 264.7 cell line with IC <subscript>20</subscript> and IC <subscript>50</subscript>  > 200 μg/ml. The extracts exhibited antioxidant and anti-inflammatory activity as evidenced by significant scavenging of ABTS and DPPH radicals and decreasing NO levels in LPS-induced RAW 264.7 cells. The antioxidant and anti-inflammatory properties of the extracts might be attributed to their phenolic and flavonoid contents. In conclusion, the traditional use of henna as a natural dye appears not to exert toxic effects and seems biosecure. Regarding safety, antioxidant, and anti-inflammatory properties, the aqueous extract of Thai henna leaf might thus serve as a readily available source for utilization in commercial health industries.
- Subjects :
- Animals
Anti-Inflammatory Agents adverse effects
Antioxidants adverse effects
Humans
Keratinocytes drug effects
Leukocytes, Mononuclear drug effects
Mice
Phytochemicals adverse effects
Plant Extracts adverse effects
Plant Extracts chemistry
Plant Leaves chemistry
RAW 264.7 Cells
Anti-Inflammatory Agents pharmacology
Antioxidants pharmacology
Lawsonia Plant chemistry
Phytochemicals pharmacology
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1528-7394
- Volume :
- 84
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of toxicology and environmental health. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 33375906
- Full Text :
- https://doi.org/10.1080/15287394.2020.1866129