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Targeting Cancer Cell Metabolism with Metformin, Dichloroacetate and Memantine in Glioblastoma (GBM).
- Source :
-
Turkish neurosurgery [Turk Neurosurg] 2021; Vol. 31 (2), pp. 233-237. - Publication Year :
- 2021
-
Abstract
- Aim: To investigate the effects of metformin, dichloroacetate (DCA), and memantine on T98G and U87-MG human glioblastoma (GBM) cells to target tumor cell metabolism in a multi-directional manner.<br />Material and Methods: IC50 levels for metformin, DCA, metformin+DCA and memantine were determined by MTT assay in T98G and U87-MG cells in vitro. Casp3, Bcl-2, Bax, c-Myc and GSK-3B protein expressions were investigated post treatments. Fifteen GBM+ tumor tissues were assessed for Casp-3, Bcl-2, Bad, Bax for apoptotic protein expression patterns.<br />Results: Cancer cell metabolism targeting drugs metformin, DCA, metformin+DCA and memantine induced cytotoxicity in a dose-dependent manner in T98G and U87-MG cells. IC50 for memantine is found as 0.5 mM (p < 0.01) which is nearly 10 times lower concentration than that of metformin. Fifteen GBM+ tumor tissues had differential apoptotic protein expressions.<br />Conclusion: Memantine exerted anti-cancer mechanism of action in T98G and U87-MG cells, however, such a mechanism requires deeper investigation for GBM treatment.
- Subjects :
- Antineoplastic Agents therapeutic use
Apoptosis drug effects
Apoptosis physiology
Brain Neoplasms drug therapy
Brain Neoplasms pathology
Cell Line, Tumor
Cell Survival drug effects
Cell Survival physiology
Dichloroacetic Acid therapeutic use
Dopamine Agents pharmacology
Dopamine Agents therapeutic use
Dose-Response Relationship, Drug
Glioblastoma drug therapy
Glioblastoma pathology
Humans
Hypoglycemic Agents pharmacology
Hypoglycemic Agents therapeutic use
Memantine therapeutic use
Metformin therapeutic use
Proto-Oncogene Proteins c-bcl-2 biosynthesis
Antineoplastic Agents pharmacology
Brain Neoplasms metabolism
Dichloroacetic Acid pharmacology
Glioblastoma metabolism
Memantine pharmacology
Metformin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1019-5149
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Turkish neurosurgery
- Publication Type :
- Academic Journal
- Accession number :
- 33372258
- Full Text :
- https://doi.org/10.5137/1019-5149.JTN.29176-20.3