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CD32 + CD4 + memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice.
- Source :
-
IScience [iScience] 2020 Nov 30; Vol. 24 (1), pp. 101881. Date of Electronic Publication: 2020 Nov 30 (Print Publication: 2021). - Publication Year :
- 2020
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Abstract
- CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4 <superscript>+</superscript> T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32 <superscript>+</superscript> memory CD4 <superscript>+</superscript> T cells were enriched in cell-associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32 <superscript>-</superscript> CD4 <superscript>+</superscript> fraction. Using multidimensional reduction analysis, several memory CD4 <superscript>+</superscript> CD32 <superscript>+</superscript> T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32 <superscript>+</superscript> CD4 <superscript>+</superscript> memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32 <superscript>-</superscript> CD4 <superscript>+</superscript> T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4 <superscript>+</superscript> T-cell subsets that contain only a small proportion of the translation-competent reservoir.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (© 2020 The Authors.)
Details
- Language :
- English
- ISSN :
- 2589-0042
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- IScience
- Publication Type :
- Academic Journal
- Accession number :
- 33364576
- Full Text :
- https://doi.org/10.1016/j.isci.2020.101881