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A dynamic understanding of cytochrome P450 structure and function through solution NMR.
- Source :
-
Current opinion in biotechnology [Curr Opin Biotechnol] 2021 Jun; Vol. 69, pp. 35-42. Date of Electronic Publication: 2020 Dec 22. - Publication Year :
- 2021
-
Abstract
- Many economically important biosyntheses incorporate regiospecific and stereospecific oxidations at unactivated carbons. Such oxidations are commonly catalyzed by cytochrome P450 monooxygenases, heme-containing enzymes that activate molecular oxygen while selectively binding and orienting the substrate for reaction. Despite the plethora of P450-catalyzed reactions, the P450 fold is highly conserved, and static structures are often insufficient for characterizing conformational states that contribute to specificity. High-resolution solution nuclear magnetic resonance (NMR) offers insights into dynamic processes and conformational changes that are required of a P450 in order to attain the combination of specificity and efficiency required for these reactions.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0429
- Volume :
- 69
- Database :
- MEDLINE
- Journal :
- Current opinion in biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 33360373
- Full Text :
- https://doi.org/10.1016/j.copbio.2020.11.007