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Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Feb 15; Vol. 170, pp. 33-41. Date of Electronic Publication: 2020 Dec 19. - Publication Year :
- 2021
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Abstract
- Fast green FCF (FGF) is often used in foods, pharmaceuticals, and cosmetics. However, little is known about the interactions of FGF with amyloid-β protein (Aβ) associated with Alzheimer's disease. In this study, the inhibitory effects of FGF on Aβ fibrillogenesis, the disruption of preformed Aβ fibrils, the reduction of Aβ-induced cytotoxicity, and the attenuation of Aβ-induced learning and memory impairments in mice were investigated. FGF significantly inhibited Aβ fibrillogenesis and disintegrated the mature fibrils as evidenced by thioflavin T fluorescence and atomic force microscopy studies. Co-incubation of Aβ with FGF greatly reduced Aβ-induced cytotoxicity in vitro. Moreover, FGF showed a protective effect against cognitive impairment in Aβ-treated mice. Molecular dynamics simulations further showed that FGF could synergistically interact with the Aβ17-42 pentamer via electrostatic interactions, hydrogen bonds and π-π interactions, which reduced the β-sheet content, and disordered random coils and bend structures of the Aβ17-42 pentamer. This study offers a comprehensive understanding of the inhibitory effects of FGF against Aβ neurotoxicity, which is critical for the search of effective food additives that can combat amyloid-associated disease.<br />Competing Interests: Declaration of competing interest The authors declare no competing financial interests.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Alzheimer Disease metabolism
Alzheimer Disease prevention & control
Amyloid drug effects
Amyloid toxicity
Amyloid ultrastructure
Amyloid beta-Peptides chemistry
Animals
Cognitive Dysfunction etiology
Cognitive Dysfunction metabolism
Exploratory Behavior drug effects
Food Additives pharmacology
Humans
Hydrogen Bonding
Lissamine Green Dyes pharmacology
Mice
Microscopy, Atomic Force
Models, Molecular
Molecular Dynamics Simulation
Morris Water Maze Test drug effects
Neuroprotective Agents pharmacology
Peptide Fragments chemistry
Peptide Fragments drug effects
Protein Aggregation, Pathological drug therapy
Protein Structure, Secondary drug effects
Random Allocation
Static Electricity
Amyloid antagonists & inhibitors
Amyloid beta-Peptides drug effects
Cognitive Dysfunction prevention & control
Food Additives therapeutic use
Lissamine Green Dyes therapeutic use
Neuroprotective Agents therapeutic use
Protein Aggregation, Pathological prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 170
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33352157
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.12.115