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Fancy-Shaped Gold-Platinum Nanocauliflowers for Improved Proton Irradiation Effect on Colon Cancer Cells.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Dec 17; Vol. 21 (24). Date of Electronic Publication: 2020 Dec 17. - Publication Year :
- 2020
-
Abstract
- Enhancing the effectiveness of colorectal cancer treatment is highly desirable. Radiation-based anticancer therapy-such as proton therapy (PT)-can be used to shrink tumors before subsequent surgical intervention; therefore, improving the effectiveness of this treatment is crucial. The addition of noble metal nanoparticles (NPs), acting as radiosensitizers, increases the PT therapeutic effect. Thus, in this paper, the effect of novel, gold-platinum nanocauliflowers (AuPt NCs) on PT efficiency is determined. For this purpose, crystalline, 66-nm fancy shaped, bimetallic AuPt NCs were synthesized using green chemistry method. Then, physicochemical characterization of the obtained AuPt NCs by transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive X-ray spectroscopy (EDS), and UV-Vis spectra measurements was carried out. Fully characterized AuPt NCs were placed into a cell culture of colon cancer cell lines (HCT116, SW480, and SW620) and a normal colon cell line (FHC) and subsequently subjected to proton irradiation with a total dose of 15 Gy. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) test, performed after 18-h incubation of the irradiated cell culture with AuPt NCs, showed a significant reduction in cancer cell viability compared to normal cells. Thus, the radio-enhancing features of AuPt NCs indicate their potential application for the improvement in effectiveness of anticancer proton therapy.
- Subjects :
- Cell Survival drug effects
Cell Survival radiation effects
Colonic Neoplasms pathology
Green Chemistry Technology
HCT116 Cells
Humans
Metal Nanoparticles chemistry
Microscopy, Electron, Transmission
Protons
Radiation-Sensitizing Agents chemistry
Spectrometry, X-Ray Emission
Colonic Neoplasms radiotherapy
Gold chemistry
Metal Nanoparticles administration & dosage
Platinum chemistry
Proton Therapy methods
Radiation-Sensitizing Agents administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33348549
- Full Text :
- https://doi.org/10.3390/ijms21249610