Back to Search
Start Over
Higher Cytokine and Opsonizing Antibody Production Induced by Bovine Serum Albumin (BSA)-Conjugated Tetrasaccharide Related to Streptococcus pneumoniae Type 3 Capsular Polysaccharide.
- Source :
-
Frontiers in immunology [Front Immunol] 2020 Dec 04; Vol. 11, pp. 578019. Date of Electronic Publication: 2020 Dec 04 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- A number of studies have demonstrated the limited efficacy of S. pneumoniae type 3 capsular polysaccharide (CP) in the 13-valent pneumococcal conjugate vaccine against serotype 3 invasive pneumococcal diseases and carriage. Synthetic oligosaccharides (OSs) may provide an alternative to CPs for development of novel conjugated pneumococcal vaccines and diagnostic test systems. A comparative immunological study of di-, tri-, and tetra-bovine serum albumin (BSA) conjugates was performed. All oligosaccharides conjugated with biotin and immobilized on streptavidin-coated plates stimulated production of IL-1 α , IL-2, IL-4, IL-5, IL-10, IFN γ , IL-17A, and TNFα, but not IL-6 and GM-CSF in monocultured mice splenocytes. The tetrasaccharide-biotin conjugate stimulated the highest levels of IL-4, IL-5, IL-10, and IFN γ , which regulate expression of specific immunoglobulin isotypes. The tetra-BSA conjugate adjuvanted with aluminum hydroxide elicited high levels of IgM, IgG1, IgG2a, and IgG2b antibodies (Abs). Anti-CP-induced Abs could only be measured using the biotinylated tetrasaccharide. The tetrasaccharide ligand possessed the highest binding capacity for anti-OS and antibacterial IgG Abs in immune sera. Sera to the tetra-BSA conjugate promoted greater phagocytosis of bacteria by neutrophils and monocytes than the CRM <subscript>197</subscript> -CP-antisera. Sera of mice immunized with the tetra-BSA conjugate exhibited the highest titer of anti-CP IgG1 Abs compared with sera of mice inoculated with the same doses of di- and tri-BSA conjugates. Upon intraperitoneal challenge with lethal doses of S. pneumoniae type 3, the tri- and tetra-BSA conjugates protected mice more significantly than the di-BSA conjugate. Therefore, it may be concluded that the tetrasaccharide ligand is an optimal candidate for development of a semi-synthetic vaccine against S. pneumoniae type 3 and diagnostic test systems.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2020 Kurbatova, Akhmatova, Zaytsev, Akhmatova, Egorova, Yastrebova, Sukhova, Yashunsky, Tsvetkov and Nifantiev.)
- Subjects :
- Animals
Antibody Specificity
Biotinylation
Cells, Cultured
Immunization
Male
Mice, Inbred BALB C
Oligosaccharides chemical synthesis
Phagocytosis drug effects
Spleen immunology
Spleen metabolism
Antibodies, Bacterial metabolism
Bacterial Capsules immunology
Cytokines metabolism
Immunogenicity, Vaccine
Oligosaccharides immunology
Pneumococcal Vaccines pharmacology
Serum Albumin, Bovine immunology
Spleen drug effects
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33343566
- Full Text :
- https://doi.org/10.3389/fimmu.2020.578019