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3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.
- Source :
-
The Journal of cell biology [J Cell Biol] 2021 Feb 01; Vol. 220 (2). - Publication Year :
- 2021
-
Abstract
- Microtubules play a major role in intracellular trafficking of vesicles in endocrine cells. Detailed knowledge of microtubule organization and their relation to other cell constituents is crucial for understanding cell function. However, their role in insulin transport and secretion is under debate. Here, we use FIB-SEM to image islet β cells in their entirety with unprecedented resolution. We reconstruct mitochondria, Golgi apparati, centrioles, insulin secretory granules, and microtubules of seven β cells, and generate a comprehensive spatial map of microtubule-organelle interactions. We find that microtubules form nonradial networks that are predominantly not connected to either centrioles or endomembranes. Microtubule number and length, but not microtubule polymer density, vary with glucose stimulation. Furthermore, insulin secretory granules are enriched near the plasma membrane, where they associate with microtubules. In summary, we provide the first 3D reconstructions of complete microtubule networks in primary mammalian cells together with evidence regarding their importance for insulin secretory granule positioning and thus their supportive role in insulin secretion.<br /> (© 2020 Müller et al.)
- Subjects :
- Animals
Cell Membrane drug effects
Cell Membrane metabolism
Cell Nucleus drug effects
Cell Nucleus metabolism
Cells, Cultured
Glucose pharmacology
Insulin metabolism
Insulin-Secreting Cells drug effects
Mice, Inbred C57BL
Microtubules drug effects
Microtubules metabolism
Secretory Vesicles drug effects
Secretory Vesicles metabolism
Mice
Imaging, Three-Dimensional
Insulin-Secreting Cells metabolism
Microscopy, Electron, Scanning
Microtubules ultrastructure
Organelles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8140
- Volume :
- 220
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 33326005
- Full Text :
- https://doi.org/10.1083/jcb.202010039