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PEGylated nano-Rehmannia glutinosa polysaccharide induces potent adaptive immunity against Bordetella bronchiseptica.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jan 31; Vol. 168, pp. 507-517. Date of Electronic Publication: 2020 Dec 10. - Publication Year :
- 2021
-
Abstract
- Vaccines, in many cases, stimulate only too weak immunogenicity to prevent infection. Therefore, adjuvants are required during their preparation to boost the immune response. We herein developed a PEGylated nano-adjuvant based on Rehmannia glutinosa polysaccharide (RGP). The addition of PEG layer exhibits enhanced immune performance of the nano-RGP. Stimulation of dendritic cells (DCs) with PEGylated nano-RGP (pRL) led to increased proliferation and cytokine production (IL-6, IL-12, IL-1β and TNF-α). The pRL was internalized into DCs via a rapid and efficient method. The mice immunized with pRL exhibited enhanced antigen-specific serum IgG and Th1-(IFN-γ), Th2-(IL-4), and Th17-(IL-17, IL-6) cytokine production, contributing to a good anti-infection performance. Furthermore, the pRL could effectively deliver the antigen to the lymph nodes (LNs), activate DC in the LN and produce enhanced CD4 <superscript>+</superscript> and CD8 <superscript>+</superscript> T-cells-derived memory (CD44 <superscript>high</superscript> CD62L <superscript>high</superscript> ), and effector (CD44 <superscript>high</superscript> CD62L <superscript>low</superscript> ) as well as functional phenotypes. Our results revealed that pRL can act as a promising adjuvant with targeted delivery of antigen due to its effective activation and robust adaptive immunity induction of DCs.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Adaptive Immunity
Adjuvants, Immunologic chemistry
Animals
Antigens, Bacterial immunology
Bacterial Vaccines administration & dosage
Bacterial Vaccines chemistry
Bacterial Vaccines immunology
CD4-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes metabolism
Cell Line
Cytokines metabolism
Dendritic Cells metabolism
Female
Immunization
Mice
Nanoparticles
Polysaccharides chemistry
Polysaccharides immunology
Adjuvants, Immunologic administration & dosage
Antibodies, Bacterial blood
Antigens, Bacterial administration & dosage
Bordetella bronchiseptica immunology
Polyethylene Glycols chemistry
Polysaccharides administration & dosage
Rehmannia chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 168
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33310103
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.12.044