Back to Search Start Over

Effects of a solid formulation containing lectin-rich fraction of Moringa oleifera seeds on egg hatching and development of Aedes aegypti larvae.

Authors :
Silva LLS
Silva SCC
de Oliveira APS
Nascimento JDS
Silva EO
Coelho LCBB
Neto PJR
Navarro DMDAF
Napoleão TH
Paiva PMG
Source :
Acta tropica [Acta Trop] 2021 Feb; Vol. 214, pp. 105789. Date of Electronic Publication: 2020 Dec 09.
Publication Year :
2021

Abstract

The measures currently used to minimize the spread of arboviruses, comprising dengue, Chikungunya, and Zika virus, involve controlling the size of population of the mosquito Aedes aegypti. However, the search for formulations containing new insecticides is gaining pace due to reports of mosquito populations showing resistance to commonly used compounds. In this study, tablets containing a protein fraction of Moringa oleifera seeds enriched in the WSMoL lectin, known to show larvicidal and ovicidal activities against A. aegypti, were developed. The compatibility between the fraction and the excipients used in obtaining the tablets was evaluated by thermogravimetry (TG), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) absorption spectroscopy. The larvicidal and ovicidal activities of the resulting tablets [5%, 10%, and 15% (w/w) of the fraction] were evaluated, as well as their effect on mosquito oviposition. Assays were also performed using a placebo tablet. According to the TG, DSC, and FTIR results, the protein composition of the fraction did not change when mixed with the components of the formulation. Tablets containing 10% and 15% WSMoL-rich fraction caused mortality of 42.5% and 95% of the larvae after 48 h, respectively, with larvae incubated with these tablets showing reduced acetylcholinesterase activity. All tablets inhibited egg hatching after 72 h (36-74%), and tablets containing 15% fraction were found to exert a repellent effect on oviposition. Our results show that the formulation developed in this study interfered with the life cycle of A. aegypti, and thus show potential for use in the control of this mosquito.<br /> (Copyright © 2020. Published by Elsevier B.V.)

Details

Language :
English
ISSN :
1873-6254
Volume :
214
Database :
MEDLINE
Journal :
Acta tropica
Publication Type :
Academic Journal
Accession number :
33309593
Full Text :
https://doi.org/10.1016/j.actatropica.2020.105789