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Nuclear Ribosomal RNA Genes: ITS Region.

Authors :
Besse P
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2222, pp. 119-129.
Publication Year :
2021

Abstract

Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ITS region of the ribosomal RNA genes remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.

Subjects

Subjects :
Base Sequence
DNA Barcoding, Taxonomic
Nucleic Acid Conformation
Phylogeny
Polymerase Chain Reaction
Reproducibility of Results
Sequence Analysis, DNA
Species Specificity
DNA, Ribosomal Spacer chemistry
DNA, Ribosomal Spacer genetics
Genes, rRNA

Details

Language :
English
ISSN :
1940-6029
Volume :
2222
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
33301091
Full Text :
https://doi.org/10.1007/978-1-0716-0997-2_7
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