Back to Search
Start Over
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus.
- Source :
-
Scientific reports [Sci Rep] 2020 Dec 08; Vol. 10 (1), pp. 21443. Date of Electronic Publication: 2020 Dec 08. - Publication Year :
- 2020
-
Abstract
- In the present work, culture-based and culture-independent investigations were performed to determine the microbiota structure of the coelomic fluid of Mediterranean sea urchin Paracentrotus lividus individuals collected from two distinct geographical sites neighboring a high-density population bay and a nature reserve, respectively. Next Generation Sequencing analysis of 16S rRNA gene (rDNA) showed that members of the Proteobacteria, Bacteroidetes and Fusobacteria phyla, which have been previously reported to be commonly retrieved from marine invertebrates, dominate the overall population of microorganisms colonizing this liquid tissue, with minority bacterial genera exhibiting remarkable differences among individuals. Our results showed that there is a correlation between microbiota structure and geographical location of the echinoderm collection site, highlighting over-representation of metagenomic functions related to amino acid and bioactive peptides metabolism in specimens inhabiting the nature reserve. Finally, we also described the developmental delay and aberrations exhibited by sea urchin embryos exposed to distinct bacterial isolates, and showed that these defects rely upon hydrophilic compound(s) synthesized by the bacterial strains assayed. Altogether, our findings lay the groundwork to decipher the relationships of bacteria with sea urchins in their aquatic environment, also providing an additional layer of information to understand the biological roles of the coelomic fluid.
- Subjects :
- Animals
Bacteria genetics
Bacteria growth & development
Bacteria isolation & purification
Bacteriological Techniques
DNA, Bacterial genetics
DNA, Ribosomal genetics
High-Throughput Nucleotide Sequencing
Microbiota
Paracentrotus microbiology
Phylogeny
Bacteria classification
Paracentrotus growth & development
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33293569
- Full Text :
- https://doi.org/10.1038/s41598-020-78534-5