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A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
- Source :
-
Molecular cell [Mol Cell] 2021 Jan 07; Vol. 81 (1), pp. 183-197.e6. Date of Electronic Publication: 2020 Dec 04. - Publication Year :
- 2021
-
Abstract
- Mre11-Rad50-Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin with the transcriptional co-regulator Mediator. MRX restricts transcription of coding and noncoding DNA by a mechanism that does not require the nuclease activity of Mre11. MRX is required to tether transcriptionally active loci to the nuclear pore complex (NPC), and it also promotes large-scale gene-NPC interactions. Moreover, MRX-mediated chromatin anchoring to the NPC contributes to chromosome folding and helps to control gene expression. Together, these findings indicate that MRX has a role in transcription and chromosome organization that is distinct from its known function in DNA repair.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Chromosomes, Fungal genetics
DNA-Binding Proteins genetics
Endodeoxyribonucleases genetics
Exodeoxyribonucleases genetics
Multiprotein Complexes metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Chromosomes, Fungal metabolism
DNA-Binding Proteins metabolism
Endodeoxyribonucleases metabolism
Exodeoxyribonucleases metabolism
Gene Expression Regulation, Fungal
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 81
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 33278361
- Full Text :
- https://doi.org/10.1016/j.molcel.2020.11.010