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Molecular phylogeny, identification and pathogenicity of Rhizopus oryzae associated with root rot of mulberry in India.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2021 Jul; Vol. 131 (1), pp. 360-374. Date of Electronic Publication: 2020 Dec 14. - Publication Year :
- 2021
-
Abstract
- Aims: Root rot caused by a group of fungi is a serious disease in mulberry. This study aims to identify and characterize Rhizopus oryzae and other fungal species associated with root rot of mulberry in India.<br />Methods and Results: Rotted root samples were collected from the mulberry gardens from four states of Southern India. The majority of the isolates identified were R. oryzae, and others were saprophytic fungi, less abundant to occasional. Two methods of inoculations were tested to confirm the pathogenicity of the selected isolates and R. oryzae was found to be pathogenic on susceptible mulberry genotypes RC2 and SRDC-1. Multi gene phylogenetic analyses using the internal transcribed spacer region (ITS), actin (ACT) and translation elongation factor 1-α (TEF), identified the isolates as R. oryzae. Additionally, Ovatospora brasiliensis, Amesia nigricolor, Gongronella butleri, Myrmecridium schulzeri, Scedosporium boydii, Graphium euwallacea, Clonostachys rosea andTalaromyces spp. were also identified.<br />Conclusion: This study revealed the existence of eleven species of fungi including the first report of R. oryzae and the occurrence of weak pathogens or saprophytes that are associated with the root rot of mulberry in India.<br />Significance and Impact of the Study: This is the first report of R. oryzae causing Rhizopus rot of mulberry in India. Moreover, the occurrence of saprophytes associated with root rot of mulberry was identified. Further studies should focus more on the ability of these species to generate secondary metabolites and extracellular lytic enzymes as they are beneficial for the management of root rot disease.<br /> (© 2020 The Society for Applied Microbiology.)
- Subjects :
- Ascomycota classification
Ascomycota isolation & purification
Fungi classification
Fungi genetics
Hypocreales classification
Hypocreales isolation & purification
India
Mucorales classification
Mucorales isolation & purification
Peptide Elongation Factor 1 genetics
Phylogeny
Plant Proteins genetics
Plant Roots microbiology
Rhizopus oryzae classification
Rhizopus oryzae genetics
Scedosporium classification
Scedosporium isolation & purification
Sordariales classification
Sordariales isolation & purification
Virulence
Fungi isolation & purification
Morus microbiology
Plant Diseases microbiology
Rhizopus oryzae isolation & purification
Rhizopus oryzae pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 131
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33277790
- Full Text :
- https://doi.org/10.1111/jam.14959