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NLRP3 and IL-1β Gene Expression Is Elevated in Monocytes From HIV-Treated Patients With Neurocognitive Disorders.
- Source :
-
Journal of acquired immune deficiency syndromes (1999) [J Acquir Immune Defic Syndr] 2021 Apr 01; Vol. 86 (4), pp. 496-499. - Publication Year :
- 2021
-
Abstract
- Background: Systemic immune activation and inflammation in chronic HIV infection are driving factors of non-AIDS-related events, including neurocognitive impairment. The role of inflammasome in monocytes from patients with HIV infection has been extensively studied, but its association with the extent of neurocognitive dysfunction has been poorly investigated.<br />Methods: We enrolled 79 HIV-positive patients; 44 with varying levels of HIV-associated neurocognitive disorder (HAND) and 35 without and 8 healthy donors. HAND subtypes included asymptomatic neurocognitive impairment (asymptomatic neurocognitive impairment; n = 19), mild neurocognitive disorder (MND; n = 17), and HIV-associated dementia (n = 8). We quantified plasmatic concentrations of proinflammatory cytokines (TNF-α, IL-6, IL-17A, IL-1β, and IFN-γ) for all HIV patients, and the mRNA expression of genes involved in the inflammasome activity (NLRP3, PYCARD, NAIP, AIM2, IL-1β, and IL-18) in monocytes of a subgroup of 28 HIV patients and 8 healthy donors.<br />Results: HIV patients' plasma concentrations of IFN-γ, IL-1β, and IL-17A were undetectable. Levels of TNF-α and IL-6 were similar among the HIV patient groups. A trend toward an increased expression of inflammasome genes according to neurocognitive disorder severity was observed. Of note, the NLRP3 mRNA relative expression was higher in MND compared with other groups, and IL-1β was lower in MND than HIV-associated dementia patients.<br />Conclusions: Changes in inflammasome components in circulating monocytes according to different HAND severity suggest that NLRP3 may be a possible biomarker or target to better understand and treat the link between systemic inflammation and neurocognitive impairment in HIV infection.<br />Competing Interests: The Iranian Research Center for HIV/AIDS (Tehran University of Medical Sciences) provided a fellowship to E.M.-T. and the International Society for Advancement of Cytometry (ISAC) the Marylou Ingram Scholarship to S.D.-B. The remaining authors have no conflicts of interest to disclose.<br /> (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.)
- Subjects :
- AIDS Dementia Complex metabolism
Adult
Anti-HIV Agents therapeutic use
Female
Gene Expression Regulation
HIV Infections drug therapy
Humans
Inflammasomes metabolism
Interleukin-1beta genetics
Male
Middle Aged
NLR Family, Pyrin Domain-Containing 3 Protein genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Viral Load
HIV Infections complications
HIV-1
Interleukin-1beta metabolism
Monocytes metabolism
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
Neurocognitive Disorders metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1944-7884
- Volume :
- 86
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of acquired immune deficiency syndromes (1999)
- Publication Type :
- Academic Journal
- Accession number :
- 33273212
- Full Text :
- https://doi.org/10.1097/QAI.0000000000002588