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A topologically distinct class of photolyases specific for UV lesions within single-stranded DNA.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2020 Dec 16; Vol. 48 (22), pp. 12845-12857. - Publication Year :
- 2020
-
Abstract
- Photolyases are ubiquitously occurring flavoproteins for catalyzing photo repair of UV-induced DNA damages. All photolyases described so far have a bilobal architecture with a C-terminal domain comprising flavin adenine dinucleotide (FAD) as catalytic cofactor and an N-terminal domain capable of harboring an additional antenna chromophore. Using sequence-similarity network analysis we discovered a novel subgroup of the photolyase/cryptochrome superfamily (PCSf), the NewPHLs. NewPHL occur in bacteria and have an inverted topology with an N-terminal catalytic domain and a C-terminal domain for sealing the FAD binding site from solvent access. By characterizing two NewPHL we show a photochemistry characteristic of other PCSf members as well as light-dependent repair of CPD lesions. Given their common specificity towards single-stranded DNA many bacterial species use NewPHL as a substitute for DASH-type photolyases. Given their simplified architecture and function we suggest that NewPHL are close to the evolutionary origin of the PCSf.<br /> (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Amino Acid Sequence genetics
Catalytic Domain genetics
Catalytic Domain radiation effects
DNA Damage radiation effects
DNA Repair radiation effects
DNA, Single-Stranded radiation effects
Deoxyribodipyrimidine Photo-Lyase radiation effects
Methylobacterium genetics
Pyrimidine Dimers genetics
Pyrimidine Dimers radiation effects
Rhodobacteraceae genetics
Ultraviolet Rays
Cryptochromes genetics
DNA, Single-Stranded genetics
Deoxyribodipyrimidine Photo-Lyase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 48
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 33270891
- Full Text :
- https://doi.org/10.1093/nar/gkaa1147