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Biodistribution, cardiac and neurobehavioral assessments, and neurotransmitter quantification in juvenile rats following oral administration of aluminum oxide nanoparticles.
- Source :
-
Journal of applied toxicology : JAT [J Appl Toxicol] 2021 Aug; Vol. 41 (8), pp. 1316-1329. Date of Electronic Publication: 2020 Dec 02. - Publication Year :
- 2021
-
Abstract
- Little is known about the uptake, biodistribution, and biological responses of nanoparticles (NPs) and their toxicity in developing animals. Here, male and female juvenile Sprague-Dawley rats received four consecutive daily doses of 10 mg/kg Al <subscript>2</subscript> O <subscript>3</subscript> NP (diameter: 24 nm [transmission electron microscope], hydrodynamic diameter: 148 nm) or vehicle control (water) by gavage between postnatal days (PNDs) 17-20. Basic neurobehavioral and cardiac assessments were performed on PND 20. Animals were sacrificed on PND 21, and selected tissues were collected, weighed, and processed for histopathology or neurotransmitter analysis. The biodistribution of Al <subscript>2</subscript> O <subscript>3</subscript> NP in tissue sections of the intestine, liver, spleen, kidney, and lymph nodes were evaluated using enhanced dark-field microscopy (EDM) and hyperspectral imaging (HSI). Liver-to-body weight ratio was significantly increased for male pups administered Al <subscript>2</subscript> O <subscript>3</subscript> NP compared with control. HSI suggested that Al <subscript>2</subscript> O <subscript>3</subscript> NP was more abundant in the duodenum and ileum tissue of the female pups compared with the male pups, whereas the abundance of NP was similar for males and females in the other tissues. The abundance of NP was higher in the liver compared with spleen, lymph nodes, and kidney. Homovanillic acid and norepinephrine concentrations in brain were significantly decreased following Al <subscript>2</subscript> O <subscript>3</subscript> NP administration in female and male pups, whereas 5-hydroxyindoleacetic acid was significantly increased in male pups. EDM/HSI indicates intestinal uptake of Al <subscript>2</subscript> O <subscript>3</subscript> NP following oral administration. Al <subscript>2</subscript> O <subscript>3</subscript> NP altered neurotransmitter/metabolite concentrations in juvenile rats' brain tissues. Together, these data suggest that orally administered Al <subscript>2</subscript> O <subscript>3</subscript> NP interferes with the brain biochemistry in both female and male pups.<br /> (© 2020 John Wiley & Sons, Ltd.)
- Subjects :
- Administration, Oral
Aluminum Oxide administration & dosage
Animals
Brain metabolism
Electrocardiography drug effects
Female
Male
Metal Nanoparticles administration & dosage
Motor Activity drug effects
Neurotransmitter Agents analysis
Rats
Rats, Sprague-Dawley
Rotarod Performance Test
Tissue Distribution
Aluminum Oxide toxicity
Heart drug effects
Metal Nanoparticles toxicity
Neurotransmitter Agents metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1263
- Volume :
- 41
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of applied toxicology : JAT
- Publication Type :
- Academic Journal
- Accession number :
- 33269475
- Full Text :
- https://doi.org/10.1002/jat.4122