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In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil.
- Source :
-
Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2020 Nov 25; Vol. 53, pp. e20200657. Date of Electronic Publication: 2020 Nov 25 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Introduction: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results.<br />Methods: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays.<br />Results: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences.<br />Conclusion: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
Details
- Language :
- English
- ISSN :
- 1678-9849
- Volume :
- 53
- Database :
- MEDLINE
- Journal :
- Revista da Sociedade Brasileira de Medicina Tropical
- Publication Type :
- Academic Journal
- Accession number :
- 33263691
- Full Text :
- https://doi.org/10.1590/0037-8682-0657-2020