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Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus-oocyte complexes: A biological and spectroscopic approach.
- Source :
-
Molecular reproduction and development [Mol Reprod Dev] 2021 Jan; Vol. 88 (1), pp. 67-79. Date of Electronic Publication: 2020 Nov 26. - Publication Year :
- 2021
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Abstract
- This study deals with the effect of plasminogen/plasmin on the in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs). Exogenous plasminogen activator streptokinase (SK) added to the IVM medium revealed similar values of cumulus expansion and oocyte nuclear maturation compared to controls (standard IVM medium). However, a decrease in both determinations was observed in COCs matured with the supplementation of ɛ-aminocaproic acid (ɛ-ACA), a specific plasmin inhibitor. After in vitro fertilization, no differences were observed in either cleavage or blastocyst rates between SK and control groups; however, ε-ACA treatment caused a decrease in both developmental rates. Zona pellucida (ZP) digestion time decreased in the SK group while it increased in the ε-ACA group. Raman microspectroscopy revealed an increase in the intensity of the band corresponding to the glycerol group of sialic acid in the ZP of oocytes matured with SK, whereas ZP spectra of oocytes treated with ɛ-ACA presented similarities with immature oocytes. The results indicate that although treatment with SK did not alter oocyte developmental competence, it induced modifications in the ZP of oocytes that could modify the folding of glycoproteins. Plasmin inhibition impairs oocyte maturation and has an impact on embryo development, thus evidencing the importance of this protease during IVM.<br /> (© 2020 Wiley Periodicals LLC.)
- Subjects :
- Aminocaproic Acid pharmacology
Animals
Blastocyst drug effects
Blastocyst metabolism
Cattle
Culture Media
Cumulus Cells drug effects
Embryo Culture Techniques methods
Embryonic Development drug effects
Female
Fertilization in Vitro methods
Fibrinolysin antagonists & inhibitors
Oocytes drug effects
Zona Pellucida drug effects
Zona Pellucida metabolism
Cumulus Cells metabolism
Fibrinolysin pharmacology
Fibrinolytic Agents pharmacology
In Vitro Oocyte Maturation Techniques methods
Oocytes metabolism
Oogenesis drug effects
Plasminogen pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-2795
- Volume :
- 88
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 33244844
- Full Text :
- https://doi.org/10.1002/mrd.23441